Postdoc CRISPR/Cas9 | Hematopoietic stem cell biology
Aarhus University, Dept. of Biomedicine
Aug. 23, 2021
About the research project
The research project will use CRISPR-based tools to study genetic programs that govern the plasticity of hematopoietic stem and progenitor cells. Increased insights into this phenomenon might bring new treatment modalities to regenerative medicine and hematological disorders. You will be using state-of-the-art CRISPRa and CRISPRi tools for transient manipulation of gene expression, and you will combine these with genome-wide screens to probe genetic mechanisms in hematopoietic stem cell biology. Ultimately, the impact of gene manipulation will be assessed in mouse transplants and hematopoietic repopulation studies.
Your job responsibilities:
The Postdoc position is primarily research-based but will also involve teaching assignments. You will contribute to the development of the department through research of high international quality.
Your main tasks will consist of:
- Cell culturing, transfection, CRISPR/Cas genetic manipulation, and lentiviral vector production.
- Genetic, phenotypic, and functional characterization of hematopoietic stem cell properties in vitro and in vivo.
- Analysis, interpretation, and presentation of research data including publication in peer-reviewed journals.
You will be under daily supervision and guidance by group leader Rasmus O. Bak (email@example.com) and you will formally report to the Head of Department, Thomas G. Jensen.
You must have academic qualifications at PhD level in medicine, molecular medicine/biology, biotechnology, or similar.
Furthermore, you must have experience in bioinformatics and performing large scale genetic screens. Ideally, you will have experience in:
- Mammalian cell culture
- Mouse handling
- Flow cytometry
- Production of viral lentiviral vectors and CRISPR/Cas9 guide libraries for genome-wide screens
You are highly motivated, scientifically curious, a self-starter, and with a proven track-record of publishing high-quality research. Excellent communication and inter-personal skills are a prerequisite. We expect you to be fluent in oral and written English.
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