You´ve Edited the Gene. Now What?
With CRISPR/Cas9 moving from a research tool into adaption as personalized medicine, safety concerns have to be addressed: an understanding of the effects on the targeted gene, but also potential off-target effects is desired.
Mass spectrometry allows for unbiased information on all detectable proteins addressing the need for understanding the overall impact of gene therapies.
A label-free mass spectrometry approach has been applied both to confirm protein level changes induced by gene editing and to measure the proteome wide changes that occur in response to the system perturbation in a single experiment. Additionally, the method does not require antibodies, making it broadly applicable to many organisms.
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