Some of the best links we picked up around the internet
By: Gorm Palmgren - Jul. 23, 2021
Top picks
In the first successful demonstration of gene knockout in a marsupial, a Japanese research team has used zygote microinjection of CRISPR-Cas9 to generate germline transmission of a mutant albino allele in opossums. The opossum is an established laboratory model and among the few mammals that can get melanoma.
Danish researchers at Novo Nordisk Foundation Centre for Protein Research have established the cryoEM structure of Cas12j3 while complexed with the R-loop formed by the crRNA and the target DNA. The structure reveals the machinery for PAM recognition, hybrid assembly and DNA cleavage. We previously wrote about Cas12j3, also known as CasΦ, in an interview with Basem Al-Shayeb.
Selective delivery of CRISPR-Cas9 to bladder cancer cells has been achieved with a chimeric adenoviral vector. The vector combines serotypes 5 and 35 and infects via CD46, a surface antigen highly expressed in bladder cancer cells.
A review by Feng Zhang and others provides a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics. The study focuses on the various assays, preamplification strategies and readouts, and highlights their main applications in sensing a wide range of molecular targets relevant to human health.
CRISPR-editing in stem cell research is the subject of a review by Taiwanese researchers. The review looks into the research design factors that affected CRISPR performance in stem cells.
The history and perspectives to the future of CRISPR are covered in a review by Slovakian researchers. The review focuses on the CRISPR system's mechanism of action, delivery methods, new progressive methods of cancer therapy and genetic modification of cells, and how to overcome technological issues.