CMN Weekly (28 December 2022) - Your Weekly CRISPR Medicine News
Some of the best links we picked up around the internet
By: Gorm Palmgren - Dec. 28, 2022
Top picks
American researchers have studied online and television news sources in the United States to determine the tone, content, and frequency in media coverage of CRISPR. They find that coverage remained ambiguous and infrequent as scientific research into CRISPR's clinical potential for treating human disease surged. This rare coverage indicates that the media still need to establish the salience of CRISPR to the degree that engages the public or policymakers. However, the issue will continue to gain importance as CRISPR transitions from experimental efforts into clinical practice.
Chinese researchers have used probiotic Lactobacillus with an electrostatically attached and ultrasound-controlled CRISPR-Cas9 system for a gene editing therapy of solid cancer tumours. The system exploits the ability of Lactobacillus to both activate the immune system and colonise the hypoxia tumour centre. When the target is reached, ultrasound releases and activates the CRISPR-Cas9 knockdown of the indoleamine 2,3-dioxygenase-1 (IDO1) gene. As a result, immunogenic cell death is amplified, and tumour immunosuppression is reversed. Furthermore, the system generates immune responses that effectively attack tumour cells in mice, contributing to the inhibition of tumour re-challenge in vivo.
Chinese researchers describe a rapid and high-throughput detection system for Helicobacter pylori nucleic acid. The system uses isothermal recombinase polymerase amplification (RPA) and CRISPR-Cas12a to detect the UreB gene with a limit of detection of 50 and 100 copies using fluorescence or lateral flow dipstick detection, respectively.
Chinese researchers review the challenges, strategies, and perspectives of amplification-free CRISPR-Cas detection technologies. The authors highlight the four main approaches to promote the performance of target amplification-free CRISPR/Cas-based technology and discuss future perspectives that will contribute to developing more efficient amplification-free CRISPR-Cas detection systems.
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