Cancer Cells Deliver CRISPR to Fight Cancer

In a remarkable study, researchers have developed a novel CRISPR-Cas9 delivery system utilising cryo-shocked lung tumour cells to target non-small cell lung cancer (NSCLC), particularly those with KRAS mutations. This innovative method, leveraging the concept of synthetic lethality, shows promise in improving the targeting efficiency of gene editing tools for cancer therapy.

By: Gorm Palmgren - Apr. 4, 2024

#CRISPRMED24

The technique involves using lung tumour cells treated with rapid liquid nitrogen shock, effectively inactivating their pathogenicity while preserving their structure and surface receptors. These cryo-shocked cells serve as carriers for CRISPR-Cas9, which is aimed at editing the CDK4 gene - a key player in the proliferation of KRAS-mutant NSCLC cells.

The delivery system capitalises on the cells' natural homing abilities to the lung, where they get captured in pulmonary capillaries and interact with endothelial cells, thereby enhancing the concentration of the therapeutic agent directly at the tumour site.

In NSCLC-bearing mice, in vivo injection of CRISPR-Cas9 plasmids packed in cryo-shocked lung cancer cells led to an almost fourfold higher concentration in the lungs compared to delivery by lipofectamine. The new CRISPR delivery system showed a significant reduction in tumour size and prolonged survival, marking an important step forward in the use of gene editing for cancer treatment.

Feng Liu from Zhejiang University, China is first author of the study that was published last week in Science Advances.

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News: Cancer Cells Deliver CRISPR to Fight Cancer
News: Cancer Cells Deliver CRISPR to Fight Cancer
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