CRISPR Off-Targets

What you will learn from this webinar

• Gene editing and the assays used to assess gene-editing outcomes such as off-target edits and chromosomal rearrangements
• How CAST-Seq can be used to assess the safety of designer nucleases
• The advantages of the CRISPECTOR tool for off-target analysis
• How CRISPECTOR increases accuracy of off-target measurements and detects adverse structural variations and translocation events that occur during genome editing.

CRISPR-Cas and related genome-editing tools have been shown to induce so called off-target editing events. Recently, researchers working with CRISPR have found that besides the small off-target insertions and deletions (indels) that are typically addressed using short read next-generation sequencing (NGS) methods, a range of on-target events may also occur after genome editing, including translocations, large deletions and chromosomal rearrangements.

Our understanding about what happens at the target site and at unintended sites throughout the genome after genome editing increases continuously, thanks to the emergence of new analytical technologies and methods. These novel approaches will enable better evaluation of the safety profile for a given therapeutic genome-editing modality.

On-demand Access here.